Samples for microbiological analyses

 

This type of sampling is technically conducted via a spot sample. Multiple rules are followed and precautions taken to avoid contaminating the collected sample.

The samples are taken according to COFRAC accreditation requirements.

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Two main sample categories

There are two main categories for this kind of sample:

Drinking water samples for analysis to check the conformity of the water consumed, Decree of 21/01/2010 (D1-type analysis), or to check the absence of health risks, Decree of 01/02/2010 (legionella).
This type of sample is collected from taps or showers in cold or hot water networks.

Samples from cooling towers (cooling towers, legionella) are taken more within a regulatory framework (Decree of 14/12/2013).

The frequency of these checks depends on the classification of your facility or your category, as well as on the results of previous measurements.

Generally, a check is carried out annually. For the cooling towers, this check may be monthly in certain cases.

 

Sampling for drinking water analysis

The aim of sampling for drinking water analysis is to detect the presence of microorganisms (bacteria, viruses and parasites) in drinking water for two reasons: to verify the effectiveness of the disinfection process, and to ensure it is safe for consumers.

The presence of microorganisms in drinking water can pose a short-term risk to consumers. The clinical presentation of the resulting pathologies is usually benign for the general population (gastrointestinal problems, diarrhoea, etc.).

 

Sampling for legionella analysis

The aim of legionella sampling and analysis is to detect the presence of legionella in facilities where these bacteria can be present and proliferate, such as in domestic hot and cold water systems, wet cooling systems or cooling towers, water features, and natural water.

UV culture of Legionella

 

Structures that must undergo sampling for microbiological analyses

This type of sampling is required for the following:

  • Collective domestic hot water production, storage and distribution installations that supply healthcare establishments, social and medical institutions, prisons, hotels, tourist accommodation and campsites, and other establishments open to the public.
  • Industrial facilities with wet cooling systems.

glass Petri dish for microbiological analysis

 

Sampling frequency

For establishments open to the public, the Decree of 1 February 2010 related to legionella monitoring in domestic hot water production, storage and distribution installations sets an annual sample testing requirement.

For industrial facilities, the Decrees of 14 December 2013 relating to the general requirements applicable to facilities covered by the declaration or registration system under heading No 2921 of the ICPE nomenclature, set the frequency at bimonthly (every 2 months) for facilities covered by the declaration system and monthly for facilities covered by the registration system.

The Decree of 11 January 2007 regarding the health inspection sampling and analysis programme for water supplied by a distribution network, pursuant to Articles R. 1321-10, R. 1321-15 and R. 1321-16 of the French Health Code, sets the sampling frequency.

 

CTC service related to sampling for microbiological analyses 

The sampling methodology varies according to the type of analysis to be conducted (D1, D2, P1, P2, RP or RS).

  • The samples are taken in accordance with the ISO 19458, FDT90-520 and FDT90-522 standards under COFRAC accreditation and by experienced staff and with absolute rigour. The rules for purging, disinfection and limiting external contamination are essential for high-quality sample collection and to ensure its representativeness.
  • The samples are stored in sterile, analytically controlled bottles designed for bacteriological analysis.
  • Our laboratory ensures transport at a controlled temperature within half a day, for immediate analysis. For this type of analysis, the shortest route possible must be taken.
  • Sending of a COFRAC-accredited report, stipulating whether or not quality the limits respect the existing standard, and listing the sample collection conditions. In certain cases, an email alert can be sent as soon as certain parameters are detected.

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